Workshop Sessions
We offer 2 days - 1 week hands-on workshop for college students in molecular biology, cell culture and Bioinformatics, Genomics and Pharmacoinformatics techniques. Projects can be started on mutually agreed dates anytime of the year.
Cell culture techniques
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Animal Cell Culture and maintenance of human cancer cell lines
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Freezing & thawing of human cancer cell lines
Screening and Evaluation of synthetic and natural compounds for anticancer activity
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Proliferation assays
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MTT assay
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LDH assay
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Colony formation assay (short-term treatment and its long-term effect)
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Spheroid assay (assay for stemness)
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Apoptosis assay (Cell death assay)
Bioinformatics analysis
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Pairwise and multiple sequence alignment and Database similarity search
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Phylogenetic tree construction and analysis
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Designing of PCR primers
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Homology modelling and threading methods
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Application of Molecular docking in computer aided drug design
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Application of molecular dynamics(GROMACS) to understand the native structures and stability of biomolecules
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Whole genome and SNP data analysis
Molecular biology techniques
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Restriction digestion
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Ligation
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DNA gel electrophoresis
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DNA gel extraction
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Preparation of competent cells from bacteria
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Bacterial transformation
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Plasmid DNA isolation from E.coli
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Genomic DNA isolation from Bacteria, animal cells, plant cells and tissue.
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Polymerase Chain Reaction (PCR)
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RNA extraction from human cancer cell lines
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cDNA synthesis
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Reverse Transcription PCR (RT-PCR)
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Induction of proliferation and differentiation in human cancer cell lines
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Protein extraction from human cancer cell lines
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SDS-PAGE and coomassie blue staining
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MTT Assay for proliferation/cytotoxicity
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Cytospin slide preparation and staining of hematopoietic cells for visualizing differentiation of cells