Workshop Sessions

We offer 2 days - 1 week hands-on workshop for college students in molecular biology, cell culture and Bioinformatics, Genomics and Pharmacoinformatics techniques. Projects can be started on mutually agreed dates anytime of the year.

Cell culture techniques

• Animal Cell Culture and maintenance of human cancer cell lines

• Freezing & thawing of human cancer cell lines

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Screening and Evaluation of synthetic and natural compounds for anticancer activity

• Proliferation assays

• MTT assay

• LDH assay

• Colony formation assay (short-term treatment and its long-term effect)

• Spheroid assay (assay for stemness)

• Apoptosis assay (Cell death assay)​

Bioinformatics analysis

• Pairwise and multiple sequence alignment and Database similarity search

• Phylogenetic tree construction and analysis

• Designing of PCR primers

• Homology modelling and threading methods

• Application of Molecular docking in computer aided drug design

• Application of molecular dynamics(GROMACS) to understand the native structures and stability of biomolecules

• Whole genome and SNP data analysis

Molecular biology techniques

• Restriction digestion

• Ligation

• DNA gel electrophoresis

• DNA gel extraction

• Preparation of competent cells from bacteria

• Bacterial transformation

• Plasmid DNA isolation from E.coli

• Genomic DNA isolation from Bacteria, animal cells, plant cells and tissue.

• Polymerase Chain Reaction (PCR)

• RNA extraction from human cancer cell lines

• cDNA synthesis

• Reverse Transcription PCR (RT-PCR)

• Induction of proliferation and differentiation in human cancer cell lines

• Protein extraction from human cancer cell lines

• SDS-PAGE and coomassie blue staining

• MTT Assay for proliferation/cytotoxicity

• Cytospin slide preparation and staining of hematopoietic cells for visualizing differentiation of cells