Workshop Sessions

We offer 2 days - 1 week hands-on workshop for college students in molecular biology, cell culture and Bioinformatics, Genomics and Pharmacoinformatics techniques. Projects can be started on mutually agreed dates anytime of the year.

Cell culture techniques

  • Animal Cell Culture and maintenance of human cancer cell lines

  • Freezing & thawing of human cancer cell lines

Screening and Evaluation of synthetic and natural compounds for anticancer activity

  • Proliferation assays

  • MTT assay

  • LDH assay

  • Colony formation assay (short-term treatment and its long-term effect)

  • Spheroid assay (assay for stemness)

  • Apoptosis assay (Cell death assay)

Bioinformatics analysis

  • Pairwise and multiple sequence alignment and Database similarity search

  • Phylogenetic tree construction and analysis

  • Designing of PCR primers

  • Homology modelling and threading methods

  • Application of Molecular docking in computer aided drug design

  • Application of molecular dynamics(GROMACS) to understand the native structures and stability of biomolecules

  • Whole genome and SNP data analysis

Molecular biology techniques

  • Restriction digestion

  • Ligation

  • DNA gel electrophoresis

  • DNA gel extraction

  • Preparation of competent cells from bacteria

  • Bacterial transformation

  • Plasmid DNA isolation from E.coli

  • Genomic DNA isolation from Bacteria, animal cells, plant cells and tissue.

  • Polymerase Chain Reaction (PCR)

  • RNA extraction from human cancer cell lines

  • cDNA synthesis

  • Reverse Transcription PCR (RT-PCR)

  • Induction of proliferation and differentiation in human cancer cell lines

  • Protein extraction from human cancer cell lines

  • SDS-PAGE and coomassie blue staining

  • MTT Assay for proliferation/cytotoxicity

  • Cytospin slide preparation and staining of hematopoietic cells for visualizing differentiation of cells