BIOTECHNOLOGY FACILITY

We offer R&D service for Biotechnolgy related studies for faculties and students of colleges and Universities. We have a well-established Genetic Engineering facility, and well-experienced faculties (both in India and abroad) as consultants. We will provide consultations and our facility for nominal fee. We will perform in genetic engineering studies on request or collaborative or mutual understanding.

I. Recombinant DNA Technology & PCR

  1. Plasmid DNA Isolation followed by agarose gel electrophoresis

  2. Restriction Digestion followed by agarose gel electrophoresis

  3. Ligation followed by agarose gel electrophoresis

  4. DNA Extraction from Agarose Gel

  5. Preparation of Competent Cells

  6. Plasmid Transformation in Competent Cells and plating on LBA plates

  7. Polymerase Chain Reaction (PCR) followed by agarose gel electrophoresis

  8. PCR primer design using software

  9. Gene cloning using PCR

  10. RNA isolation from bacteria / yeast / animal cells

  11. Quality check of RNA using spectroscopy

  12. Gene expression studies using RT-PCR

  13. Construction of recombinant expression plasmids

  14. Recombinant protein production in E.coli using IPTG

  15. Analysis of gene expression using Green Flourescent Protein

  16. Separation of proteins using SDS PAGE

  17. Purification of recombinant proteins using affinity chromatography

  18. Assay of recombinant protein to check purity

  19. Protein desalting using dialysis

  20. Concentration of proteins using spin filter membranes

II. Short-term training on Genetic Engineering techniques

We impart training on Genetic engineering techniques to person according to their research and academic needs.