
BIOTECHNOLOGY FACILITY
We offer R&D service for Biotechnolgy related studies for faculties and students of colleges and Universities. We have a well-established Genetic Engineering facility, and well-experienced faculties (both in India and abroad) as consultants. We will provide consultations and our facility for nominal fee. We will perform in genetic engineering studies on request or collaborative or mutual understanding.
I. Recombinant DNA Technology & PCR
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Plasmid DNA Isolation followed by agarose gel electrophoresis
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Restriction Digestion followed by agarose gel electrophoresis
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Ligation followed by agarose gel electrophoresis
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DNA Extraction from Agarose Gel
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Preparation of Competent Cells
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Plasmid Transformation in Competent Cells and plating on LBA plates
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Polymerase Chain Reaction (PCR) followed by agarose gel electrophoresis
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PCR primer design using software
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Gene cloning using PCR
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RNA isolation from bacteria / yeast / animal cells
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Quality check of RNA using spectroscopy
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Gene expression studies using RT-PCR
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Construction of recombinant expression plasmids
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Recombinant protein production in E.coli using IPTG
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Analysis of gene expression using Green Flourescent Protein
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Separation of proteins using SDS PAGE
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Purification of recombinant proteins using affinity chromatography
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Assay of recombinant protein to check purity
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Protein desalting using dialysis
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Concentration of proteins using spin filter membranes
II. Short-term training on Genetic Engineering techniques
We impart training on Genetic engineering techniques to person according to their research and academic needs.